https://sourceforge.net/p/prinseq/support-requests/2012-01-20T20:08:49ZRecent changes to support-requests2012-01-20T20:08:49Z2012-01-20T20:08:49ZAnonymoushttps://sourceforge.net/u/userid-None/https://sourceforge.net8a193c1e95d578da4f7d5621e087d442baa5b7bf

<div class="markdown_content"><p>I am working with illumina metatranscriptomic fastq files at around 8 GB. The vast majority of my exact duplicates are ribosomal sequences that I would like to keep in my quality filtered dataset with prinseq. Is there a way to turn off the duplicate removal in the standalone version?</p></div>

2011-10-18T07:06:10Z2011-10-18T07:06:10ZAnonymoushttps://sourceforge.net/u/userid-None/https://sourceforge.net39a018969bc43890a78b83670cf5aae653837c52

<div class="markdown_content"><p>I really really like your site. I've a rather silly question though. After uploading a fasta file I get all the stats but I don't understand how to run the file with the filter and trim options. So I'd like to analyze a fasta file and then do some seperate sessions of trimming.<br /> best regards and thanks foor your support!</p></div>

2011-10-13T13:21:48Z2011-10-13T13:21:48ZJavier Alonsohttps://sourceforge.net/u/fjalonso1234/https://sourceforge.netcf8a0cc806ebd44c567f94e6076f66a5e0897153

<div class="markdown_content"><p>We are using prinseq-lite to filter fastq files with the parameters -derep 1. The function works fine with files less that 3 GB, but I have problems when filtering largest files (I think that the computer is probably out of memory). I have a computer with a i7 intel processor and 16 GB RAM. The host OS is Windows 7 and I run prinseq on a Virtual Machine (VMware) loaded with Centos 5. I have assigned 8 GB RAM to the Virtual Machine. Could it be a problem of memory?<br /> Thank you very much in advance</p></div>