Recent changes to support-requests

Turn off duplicate removal

Anonymous — Fri, 20 Jan 2012 20:08:49 -0000

I am working with illumina metatranscriptomic fastq files at around 8 GB. The vast majority of my exact duplicates are ribosomal sequences that I would like to keep in my quality filtered dataset with prinseq. Is there a way to turn off the duplicate removal in the standalone version?

processing after analysis on webversion

Anonymous — Tue, 18 Oct 2011 07:06:10 -0000

I really really like your site. I've a rather silly question though. After uploading a fasta file I get all the stats but I don't understand how to run the file with the filter and trim options. So I'd like to analyze a fasta file and then do some seperate sessions of trimming.
best regards and thanks foor your support!

problems with large files

Javier Alonso — Thu, 13 Oct 2011 13:21:48 -0000

We are using prinseq-lite to filter fastq files with the parameters -derep 1. The function works fine with files less that 3 GB, but I have problems when filtering largest files (I think that the computer is probably out of memory). I have a computer with a i7 intel processor and 16 GB RAM. The host OS is Windows 7 and I run prinseq on a Virtual Machine (VMware) loaded with Centos 5. I have assigned 8 GB RAM to the Virtual Machine. Could it be a problem of memory?
Thank you very much in advance