The plates do not contain 'standard'-type wells.The standard curves can't be proceeded.

Hector A. Lucero — Fri, 03 Jan 2020 00:55:48 -0000

My A&B 7900 HT produces a number txt files when exporting data: Background spectrum.txt, Pure spectrum.txt, table.txt and a raw spectrum folder containing 384 txt files for each well number (not well coordinates). pyQPCR gives me an error: "....does not contain any well in the right format" . Questions are: 1) Which file should I use to be processed by pyQPCR and 2) What's the point A&B 7900 HT giving the option of exporting a folder with 384 files that cannot be interpreted to extract data. Thanks for your help and apologies for my illiteracy!

Hector

Esco Swift™ Spectrum 48 Real Time Thermal Cyclers

Y.B. Alahapperuma — Wed, 29 Aug 2018 09:25:21 -0000

I'm using Esco Swift™ Spectrum 48 Real Time Thermal Cyclers for my reseach works andI need to know how can I save or export dataf ile from my cycler that to support with this software? Also I expect any refference matiral or guide wiki better than available with this. Thank you.

RotorGene6000

Grosboillot Virginie — Wed, 21 Feb 2018 10:36:51 -0000

Hey there,
I am working with Rotor Gene 6000. I tried to import my files with QIAGEN Corbett but it doesn't work. I've tried all the .csv files available but none of them works. Could you tell me if this input file format is compatible with this device? And if not is it possible to create an imput format specific to RotorGene qPCR devices?
Thank you.

Using ABI 7300, and I can not import file

Steffen Buessecker — Mon, 12 Jun 2017 22:40:41 -0000

Dear Thomas,
May I send you my txt file as well? We are using an Applied Biosystem Sciences 7900 machine. I would really love to use the software and would be very grateful for any help or comment on how I need to change my txt file to be able to import.
cheers,
steffen

LightCycler480 file export

Steffen Buessecker — Mon, 12 Jun 2017 22:38:00 -0000

Hello everybody,
I am having the same import issues like many others. We are using an Applied Biosystem Sciences 7900 machine. I would really love to use the software and would be very grateful for any help or comment on how I need to change my txt file to be able to import.
cheers,
steffen

LightCycler480 file export

methylating DNA — Wed, 18 Jan 2017 16:57:03 -0000

Dear All,
I recently began doing qPCR and found your program online. I intent to do basic relative quant.
However, there's a catch: I have not been able to open any of the files I have exported from the LC480 (v1.5). Almost every error message that has been posted in this forum has appeared in my screen. I have exported in every posible way (.txt, .ixo, *.xml) almost everything that has crossed my path (tables, whole sheets, results, sample editor, analysis sheets, whole document) in both the sample editor and the analysis tabs of the LC480.
I did manage to open a table from the sample editor, but there are no Cp values there. No analysis has been done there, as it only contains the sample arrangement.

Could someone please indicate me which is the the actual sheet/table that must be exported to make this work?

thanks

Welcome to New file format

Norde — Fri, 08 Jul 2016 09:21:32 -0000

Hello Thomas,
First of all, thank you for development of pyQPCR. We used a lot this software, me and my colleagues!

Do you have some news about compatibility with Biorad CFX96 ? Do you need otherdata output or support of this device is not possible?

Best regards,
Norde

LC 480 xml file

laurent manchon — Thu, 09 Jun 2016 10:41:11 -0000

--Hi,

i don't know why i can't open my xml file, I use ROCHE LC 480 and i export file to xml format.
this the traces from the pyQPCR log file:

Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 629, in closePlate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 629, in closePlate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 629, in closePlate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 629, in closePlate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 629, in closePlate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 629, in closePlate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 629, in closePlate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 629, in closePlate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1519, in disable
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1519, in disable
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1491, in enable
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1519, in disable
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1135, in applyModel
File "pyQPCR\dialogs\modelDialog.pyc", line 113, in init
File "pyQPCR\dialogs\modelDialog.pyc", line 128, in populateImgModel
File "pyQPCR\utils\odict.pyc", line 424, in getitem
KeyError: PyQt4.QtCore.QString(u'')
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1519, in disable
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1135, in applyModel
File "pyQPCR\dialogs\modelDialog.pyc", line 113, in init
File "pyQPCR\dialogs\modelDialog.pyc", line 128, in populateImgModel
File "pyQPCR\utils\odict.pyc", line 424, in getitem
KeyError: PyQt4.QtCore.QString(u'')
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 629, in closePlate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1519, in disable
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1168, in editWell
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1168, in editWell
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'
Traceback (most recent call last):
File "pyQPCR\mainWindow.pyc", line 1336, in extractSubplate
AttributeError: 'Qpcr_qt' object has no attribute 'currentPlate'

is somebody can help me please ?

thank you --

Using ABI 7300, and I can not import file

Thomas Gastine — Wed, 04 Nov 2015 20:53:50 -0000

Send me an example CSV of TXT file which doesn't work, I'll try to figure out what's going out.

Welcome to New file format

Thomas Gastine — Wed, 04 Nov 2015 20:52:38 -0000

Dear Kelly,
You can try to send me one file by email (csv or txt) I'll see if I can include it.
Thomas

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