Comics_Calibr

Calibr is a tool for the spectrum-centric analysis of DIA data against DDA-based spectral library, to perform optimization of spectrum preprocessing and calculating various spectral similarity measures as features for validation. In addition to spectral similarity measures, Calibr provides other two types of features related to precursor ion and spectrum property and statistics from spectrum-spectrum matches (SSMs) for machine-learning based validation. To support users to conveniently conduct spectral library searching and validation, we have also implemented a GUI application, called CalibrWizard. It integrates Calibr and PercolatorTsvConverter, a conversion tool for converting Calibr's results into a tab-delimited format as input to Percolator, and can use the function of Percolator for validation. Using the wizard, users can conveniently set the parameters of the three tools and execute the workflow. Our website: https://ms.iis.sinica.edu.tw/comics/Software_CalibrWizard.html